PE anti-human CD8a Antibody

Product Details

Isotype Control

PE Mouse IgG1, κ Isotype Ctrl

Verified Reactivity

Human

Reported Reactivity

Chimpanzee, Common Marmoset

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Clone HIT8a recognizes the alpha chain of CD85. It does not block the binding of RPA-T8 antibody to CD8a.

Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections5,6. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.

Application References

(PubMed link indicates BioLegend citation)

1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
4. Awasthi, S., et al. 2011. J. Virol 85:10472. PubMed
5. Coppieters KT, et al. 2012. J. Exp. Med. 209:51. (IHC, epitope)
6. Suzuki F, et al. 2012. Arthritis Res. Ther. 14:R48. (IHC)

Isotype Control

PE Mouse IgG1, κ Isotype Ctrl

Product Citations

1. Jiang XL, et al. 2021. Nat Commun. 12:897. PubMed
2. Tatari N, et al. 2022. Front Immunol. 13:905768. PubMed
3. Cao B, et al. 2022. Nat Commun. 13:6203. PubMed
4. Ali A, et al. 2020. J Infect Dis. 222:853. PubMed
5. Zhu W, et al. 2021. Onco Targets Ther. 14:2149. PubMed
6. Dushyanthen S, et al. 2017. Nat Commun. 10.1038/s41467-017-00728-9. PubMed
7. Vereertbrugghen A, et al. 2021. Front Oncol. 11:598319. PubMed
8. Chulpanova DS, et al. 2020. Bioengineering (Basel). 7:00. PubMed
9. Wang Y, et al. 2021. Sci Rep. 1.429861111. PubMed
10. Dockry E, et al. 2018. Oncoimmunology. 7:e1428156. PubMed
11. Dziedzic K, et al. 2021. International Immunopharmacology. 96:107645. PubMed
12. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed