Product Details

Isotype Control

FITC Mouse IgG1, κ Isotype Ctrl (ICFC)

Isotype Control

FITC Mouse IgG1, κ Isotype Ctrl

Verified Reactivity

Human

Reported Reactivity

Chimpanzee, Baboon, Cynomolgus, Rhesus, African Green, Pigtailed Macaque, Sooty Mangabey

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

E. coli-expressed recombinant human IFN-γ

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser

Blue Laser (488 nm)

Application Notes

Flow Cytometry2: The fluorochrome-labeled B27 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-? -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.
Neutralization1,3,6,7: The Ultra-LEAF™ Purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IFN-? bioactivity (Cat. No. 506531).

Application References

(PubMed link indicates BioLegend citation)

1. Favre C, et al. 1989. Molec. Immunol. 26:17. (Neut)
2. Kaur A, et al. 2002. J Virol. 76:3646.
3. Abrams J, et al. 1992. Immunol. Rev. 127:5. (Neut)
4. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
5. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
6. Acosta-Rodriguez EV, et al. 2007. Nat. Immunol. 9:942-9. (Neut)
7. Gangur V, et al. 1998. FASEB J. 12:705-13. (Neut)